| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tantsyura, Eva D (12)
|
MRUN-S |
42.27 |
36.38 |
-5.89 |
| 2 |
Trieff, Lily S (12)
|
MRSS |
37.93 |
36.51 |
-1.42 |
| 3 |
Mainiero, Sophia L (12)
|
MRSS |
37.05 |
36.65 |
-0.40 |
| 4 |
Barabasi, Bianca R (11)
|
MRFAST |
39.91 |
39.36 |
-0.55 |
| 5 |
Punnoose, Ann J (11)
|
MRFAST |
40.99 |
39.69 |
-1.30 |
| 6 |
Silver, Christina M (12)
|
MRSS |
42.29 |
40.29 |
-2.00 |
| 7 |
Phillips, Maya C (12)
|
MRUN-S |
48.11 |
40.57 |
-7.54 |
| 8 |
O'Flaherty, Roisin (11)
|
MRSS |
48.61 |
43.47 |
-5.14 |
| 9 |
Skjeveland, Kira R (12)
|
MRFAST |
50.03 |
46.52 |
-3.51 |
|
Lenskis-Kristian, Michaela B (12)
|
MRFAST |
|
NS |
- |